Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China.

Shuhong Zhao, professor

Shengsong Xie, associate professor
Email: xie_ss@aliyun.com

Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan 430070, P. R. China.

Yaping Fang, associate professor
Email: ypfang@mail.hzau.edu.cn

We have developed several software/databases, we hope you enjoy them.

For sncRNAs assay:

1. sRNAPrimer v1.0: http://www.animalgenome.org/cgi-bin/host/sRNAPrimer/d

2. sRNAPrimer v2.0: http://123.57.239.141:8080/

For CRISPR/Cas9-Based Genome Editing assay:

1. sgRNAcas9: http://www.biootools.com/

Xie S, Shen B, Zhang C, Huang X, Zhang Y. sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One. 2014, 9(6):e100448. doi: 10.1371/journal.pone.0100448.

2. CRISPR-offinder: https://sourceforge.net/projects/crispr-offinder-v1-2/

Zhao C, Zheng X, Qu W, Li G, Li X, Miao Y, Han X, Liu X, Li Z, Ma Y, Shao Q, Li H, Sun F*, Xie S*, Zhao S*. CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif. Int J Biol Sci, 2017,13(11):1470-1478.

3. CRISPRamplicon: https://sourceforge.net/projects/crispramplicon/