sRNAPrimerDB -- Official Site

Small ncRNA sequences (fasta format) [Example]

Background DNA

Background DNA are DNAs which may interfere with your target template sequences in your sample.

RT primer

Specific Length (bp)

Opt

Gap Length (bp)

Cutoff

Optimal Tm (°C)

Opt

Tm cutoff (°C)

Cutoff

Max difference Tm(°C)

Diff

Primer size (bp)

Min - Max

Primer GC (%)

Min - Max

Concentration of monovalent cations (usually KCl)

mM

Concentration of divalent cations (usually MgCl₂)

mM

Concentration of dNTPs

mM

Annealing oligo concentration

nM